ESCHERICHIA COLI EXPRESSION VECTOR AND THE USES THEREOF

摘要

The present invention discloses Escherichia coli expression vector, as well as a method for steadily, high-efficiently expressing a required target protein in Escherichia coli using the expression vector. The expression vector is constructed from vector pRSET-A, wherein ampicillin resistance gene in said vector pRSET-A is replaced by kanamycin resistance gene. The expression vector can be used to high-efficiently express enzyme required for the production of 7-aminocephalosporanic acid, meanwhile it does not produce ß-lactamase by which the productivity of 7-aminocephalosporanic acid is reduced. The present invention also discloses two expression vectors, i.e., one expression vector pHS-GHA whose gene product is D-amino acid oxidase mutant GHA, and another expression vector pT7-kan-ACY whose gene product is glutaryl-7-aminocephalosporanic acid acylase of Pseudomonas sp. SE83.