Method for simulating a production process of a substance

摘要

A method for simulating a substance production process using cells based on differential equations concerning intracellular metabolites and gene expression comprising preparing the differential equations concerning intracellular metabolites and gene expression, assigning values for parameters in the differential equations required for the simulation and solving the differential equations with the assigned values, which is characterized by the following (a) to (d): (a) the differential equations include a differential equation including a specific growth rate of the cells, and the specific growth rate is represented as a function of time; (b) all or a part of parameters of the differential equations are represented as a function of the specific growth rate or a function of time; (c) the differential equations include a formation rate for formation of a cell component from an intracellular metabolite, and the formation rate is represented as a function of the specific growth rate or a function of time; and (d) the differential equations include an inflow rate of a metabolite taken up from the outside of the cells and/or an outflow rate of a metabolite excreted out of the cells from the inside of the cells, and the inflow rate and/or the outflow rate is represented as a function of the specific growth rate or a function of time.