NOVEL HIV-BASED RECOMBINANT VIRAL CLONES AND USE THEREOF IN ANALYTICAL METHODS

摘要

The present invention refers to HIV-based recombinant viral clones that possess the general structure represented in figure 8 and are the result of the following genetic manipulations:
- deletion of HIV fragments (for example, Nef gene) without losing infective capacity,
- insertion of a non-expressed gene in human cells,
- insertion of LacZ gene,
introduction of restriction sites for extracting DNA fragments of matrix provirus and substituting them for genes from patients to assess. The present invention also refers to the application of these clones in analytical methods related Lo AIDS.