RAPID ASSAYS FOR THE ASSESSMENT OF ORGAN STATUS BASED ON THEDETECTION OF ONE OR MORE ISOENZYMES OF GLUTATHIONE S-TRANSFERASE

摘要

A method for the rapid assessment of organ status, including organ damage following immunological or toxicological insult, in a subject based on the detection of one or more isoenzymes of glutathione S-transferase (GST) in diverse biological fluids comprises contacting a particle-labelled anti-GST antibody specific for said isoenzyme with a sample of a biological fluid suspected of containing said isoenzyme, said antibody having a sensitivity sufficient to detect at least at picomolar amount of said isoenzyme, and capturing the particle-labelled antibody-isoenzyme complex on an immobilised capture antibody to generate a visually detectable signal. The method permits the detection or determination of GST isoenzymes typically in a time of the order of 30 minutes from initial contact with immobilised anti-GST antiboby. The method is simple to use and thus can be used, for example, by patients at home to monitor their levels of a particular GST isoenzyme following a liver or renal transplant.