METHOD FOR ENHANCING TWO-DIMENSIONAL GEL ELECTROPHORESIS RESOLUTION

摘要

One aspect of the present disclosure is directed to compositions and methods to decrease streaking on two-dimensional gels and enhance the resolution of proteins in the gel. In accordance with one embodiment the polypeptides are contacted with a trialkylphosphine to reduce the polypeptides, followed by alkylation with a compound that provides greater than 95% alkylation of the polypeptides. The resulting alkylated polypeptides are then subjected to two-dimensional gel analysis.