Nucleoside cyanine dye derivatives for RNA labeling and nucleic acid detection and methods for using same

摘要

Fluorescent adenosine derivatives that can be incorporated into the 5' end of transcribed RNA are disclosed. Adenosine derivatives have readily detectable cyanine dyes attached. Symmetric derivatives are disclosed that. The transcribed RNAs can be used in a variety of applications including nucleic acid detection and in the study of RNA structure and function. Methods of generating labeled RNAs from a biological sample also are disclosed.