Serum free and animal protein free culture medium for cultivation of cells

摘要

Described is an animal protein free cell culture medium which is capable of supporting growth of animal cells. The culture medium comprises a purified soy hydrolysate in a concentration of between about 0.2% (w/v) to about 0.6% (w/v) and a purified yeast hydrolysate in a concentration of between about 0.05% (w/v) to about 0.2% (w/v) and at least 90% of the hydrolysates have a molecular weight of less than or equal to 1000 Daltons. Also described is a method of producing the animal protein free cell culture medium capable of supporting the growth of animal cells, a method of cultivating an animal cell culture, an animal protein confluent cell culture process, a culture of animal cells cultivated in the animal protein free medium and a method for producing a virus.