METHODS AND COMPOSITIONS TO DETECT BACTERIA USING MULTIPLEX PCR

摘要

A novel multiplex polymerase chain reaction assay for detecting a bacteria (e.g. Helicobacter pylori) in a specimen uses multiple oligonucleotide primer pairs based on the sequences of multiple loci of the bacteria. In one application, up to five loci in the genomic DNA sequences of Helicobacter pylori were amplified. Two fragments of H. pylo were amplified from each locus, wherein a second fragment was an internal fragment of the first fragment.