METHODS AND KITS FOR IDENTIFYING NUCLEIC ACID SEQUENCES

摘要

Methods and kits for detecting a nucleic acid sequence-of-interest in a sample are provided. The methods and kits use RecA-mediated strand exchange which is sensitive to a single-mismatch and which can exchange strands of non-denatured polynucleotide sequences of a sample. Thus the methods and kits of the present invention can be used for the identification of single nucleotide polymorphisms (SNPs), length of telomeric ends, numeration of chromosomes and disease-associated genes in a sample.