METHOD FOR THE PURIFICATION OF G-CSF

摘要

The invention relates to a method for obtaining the recombinant granulocyte- colony-stimulating factor (G-CSF), said method involving at least one cation- exchange chromatography step and at least one hydrophobic interaction chromatography step, the two chromatography steps directly succeeding each other in any order. The invention especially relates to a method for purifiyng G-CSF using a mixture of G-CSF and other proteins, said method comprising two cation-exchange chromatography steps which are respectively carried out before and after a hydrophobic interaction chromatography step.