| 摘要 |
Apparatus and method for determining the identity of a sequence of chemical groups in a linear polymer sample, such as the sequence of bases in a nucleic acid are disclosed. The apparatus has a substrate having a mirror surface which is formed of a plasmon resonant metal, a source of a beam of light, and a lens assembly composed of one or more plasmon resonance particles disposed about an opening defining a detection region. The particles arranged to produce, when the light beam is directed onto a sample at the detection region, near-field electromagnetic gap modes in a space between the nanolens and a confronting detection region on the substrate surface, in a gapbetween the nanolens and substrate having a selected spacing of 40 nm or less. Also included in the apparatus are a detector for receiving light emitted by or scattered from the sample at the detection region, and for converting the received light into a gap-mode enhanced Raman spectrum, and a translation mechanism for moving the sample relative to lens assembly, through the opening in the lens assembly, to position successive chemical groups in the sample at the detection region. |
