| 摘要 |
This invention relates to noninvasive methods of early detection of different sicknesses, like precancerous state or early stages of cancer development, pathologies of pregnancy, and monitoring of efficacy of anticancer therapy. The method is based on cell-surface extra-cellular nucleic acids from human blood which is divided into plasma and cellular fractions, and further divided into Leukocytes and erythrocytes. Cell-Surface-bound extra-cellular nucleic acids are eluted form cell surface with PBS-EDTA treatment or treatment of cells with trypsin solution. Eluted nucleic acids are isolated with convenient method and analyzed for presence of at least two specific sequences of nucleic acids such as PCR analysis, multiplex PCR, hybridization assay or other methods, thereby increasing the reliability of early detection of the diseases with abnormal functioning of genetic apparatus of cells due to increase of sensitivity of detection of specific DNA and RNA sequences.
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