发明名称 |
HYBRID TETANUS TOXOID PROTEINS THAT MIGRATE RETROGRADELY AND TRANSYNAPTICALLY INTO THE CNS |
摘要 |
<p>The non-toxic proteolytic C fragment of tetanus toxin (TTC peptide) has the same ability to bind nerve cells and be retrogradely transported through a synapse as the native toxin. A hybrid protein encoded by the lacZ-TTC gene fusion retains the biological functions of both proteins in vivo, i.e. retrograde transynaptic transport of the TTC fragment and -gal enzymatic activity. After intramuscular injection, enzymatic activity could be detected in motoneurons and connected neurons of the brainstem areas. This strategy is useful for the delivery of a biological activity to neurons from the periphery to the central nervous system. Such a hybrid protein can also be used to map synaptic connections between neural cells.</p> |
申请公布号 |
EP1049712(B1) |
申请公布日期 |
2007.01.24 |
申请号 |
EP19980945222 |
申请日期 |
1998.08.12 |
申请人 |
INSTITUT PASTEUR |
发明人 |
COEN, LAURENT;OSTA PINZOLAS, ROSARIO;BRULET, PHILIPPE |
分类号 |
C07K14/33;A61K38/00;A61K38/46;A61K39/08;A61K47/48 |
主分类号 |
C07K14/33 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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