发明名称 |
Generation of single-strand circular DNA from linear self-annealing segments |
摘要 |
The present invention provides a method for the rapid simultaneous production of a plurality of single-stranded DNA circles having a predetermined size and nucleotide sequence using pre-designed hairpin oligonucleotides containing complementary sequences for directing ligation to form dumbbell-shaped monomers followed by heat denaturation to yield single-stranded DNA circles.
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申请公布号 |
US2007015182(A1) |
申请公布日期 |
2007.01.18 |
申请号 |
US20060429549 |
申请日期 |
2006.05.05 |
申请人 |
ABARZUA PATRICIO |
发明人 |
ABARZUA PATRICIO |
分类号 |
C12N15/09;C12Q1/68;C12N15/10;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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