| 摘要 |
A positive displacement pump design that allows optical analysis of the fluids being pumped, mixed or sorted, via optical detection within the pump itself. Various embodiments of the device are disclosed, one employing a glass rod as a piston that gives the ability to convey optical light into the pump cylinder and collect light back out. Another employs a piston rod that encapsulates one or more optical fibers coupled to a gradient index lens at the distal end of the piston rod. A third embodiment comprises an optical fiber that serves directly as a piston actuator rod inserted through the capillary channel of a ceramic ferrule that slidably seats and aligns the optical fiber/piston actuator rod. In all embodiments the pump and detection system can be controlled and synchronized by a microprocessor, and optical detection can be based on fluorescence, scattered light, absorbance, or both.
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