发明名称 |
Parallel polymorphism scoring by amplification and error correction |
摘要 |
This invention provides a method of detecting polymorphisms, e.g., single nucleotide polymorphisms (SNPs), by amplification and error correction. The invention encompasses methods of performing amplification and error correction using an improved generation of nucleic acid polymerases, and methods of multiplexing the assay. The improvement to the polymerases is the joining of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
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申请公布号 |
US2007009954(A1) |
申请公布日期 |
2007.01.11 |
申请号 |
US20060521754 |
申请日期 |
2006.09.15 |
申请人 |
BIO-RAD LABORATORIES, INC. |
发明人 |
WANG YAN;FINNEY MICHAEL;CHEN FAN |
分类号 |
C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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