| 摘要 |
The invention provides a vector having a nucleic acid operably linking a promoter, an intron, a secretory leader sequence encoding nucleic acid, a human betacellulin (BTC) encoding nucleic acid, or functional fragment thereof, and a polyadenylation signal sequence, wherein expression of BTC produces a secreted, mature BTC. Also provided is a vector having a nucleic acid operably linking a cytomegalovirus (CMV) promoter and enhancer region, a beta-globin chimeric intron, an albumin leader sequence encoding nucleic acid, a human betacellulin (BTC) encoding nucleic acid, or a functional fragment thereof, and an SV40 polyadenylation signal sequence, wherein expression of BTC produces a secreted, mature BTC. A host cell containing the vectors of the invention are also provided. A method of treating or preventing diabetes is further provided. The method includes administering to an individual an effective amount of a viral particle having a vector expressing a secreted, mature human betaculin (BTC) or a functional fragment thereof, the vector comprising a nucleic acid operably linking a promoter, an intron, a secretory leader sequence encoding nucleic acid, a human betacellulin (BTC) encoding nucleic acid, or functional fragment thereof, and a polyadenylation signal sequence.
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