FUNCTIONAL BIOLUMINESCENCE ENERGY RESONANCE TRANSFER (BRET) ASSAY TO SCREEN, IDENTIFY AND CHARACTERIZE RECEPTOR TYROSINE KINASE LIGANDS

摘要

Disclosed herein are methods of evaluating whether a test compound functions as a ligand for a receptor tyrosine kinase. Also provided are methods of making pharmaceutical compositions that include agonists, antagonists, or inverse agonists of receptor tyrosine kinases. Also provides are isolated cells that include a receptor tyrosine kinase and a second protein, wherein the receptor tyrosine kinase contains a fluorescent donor moiety and the second protein contains a fluorescent acceptor moiety.