发明名称 |
Arginine hydrochloride enhances chaperone-like activity of alpha crystallin |
摘要 |
The present invention provides that arginine, a biologically compatible molecule that is known to bind to the peptide backbone and negatively charged side-chains, increases the chaperone-like activity of a-crystallin significantly. Arginine, interestingly, restores the activity of mutant protein to a considerable extent. The invention shows the effect of arginine on the structural changes of a-crystallin by circular dichroism, fluorescence and glycerol gradient sedimentation. The invention shows that arginine brings about subtle changes in the tertiary structure and significant changes in the quaternary structure of a-crystallin and enhances its chaperone activity significantly. This invention thus has role in designing strategies to improve chaperone function for therapeutic applications.
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申请公布号 |
US2007009956(A1) |
申请公布日期 |
2007.01.11 |
申请号 |
US20030528443 |
申请日期 |
2003.12.30 |
申请人 |
COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH |
发明人 |
SRINIVAS VOLETY;RAMAN BAKTHISARAN;RAO KUNCHALA;RAMAKRISHNA TANGIRALA;RAO CHINTALAGIRI |
分类号 |
G01N33/53;A61K31/197;A61K38/28;C07K14/47;C12Q1/37 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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