发明名称 |
PUERARIA THUNBERGIANA EXTRACT HAVING AN ACTIVITY OF ACTIVATING CHOLINE ACETYLTRANSFERASE IN BRAIN NERVE CELLS |
摘要 |
The extract of Pueraria thunbergiana or a low molecular weight flavonoid extracted therefrom is provided to activate choline acetyltransferase in brain nerve cells, so that it is useful for preventing or treating brain nerve disease. A composition for activating choline acetyltransferase in brain nerve cells comprises the extract of Pueraria thunbergiana as an effective ingredient, wherein the extract of Pueraria thunbergiana is prepared with methanol or ethanol. The low molecular weight flavonoid extracted from the extract of Pueraria thunbergiana is 4',7-dihydroxyisoflavone(daidzein), wherein low molecular weight flavonoid is purified by (i) extracting dried Pueraria thunbergiana with methanol or ethanol, (ii) fractionating the methanol or ethanol extract with hexane, chloroform and ethylacetate sequentially, (iii) subjecting the ethylacetate fraction to first and second open silica gel column, (iv) subjecting the active fraction to thin layer chromatography, and (v) subjecting the active fraction to high pressure liquid chromatography.
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申请公布号 |
KR20070002279(A) |
申请公布日期 |
2007.01.05 |
申请号 |
KR20050057720 |
申请日期 |
2005.06.30 |
申请人 |
KOREA UNIVERSITY INDUSTRY AND ACADEMY COOPERATIONFOUNDATION |
发明人 |
SHIN, DONG HOON;CHO, HONG YOUN;HONG, BUM SHIK;KIM, HYE KYUNG;KIM, MI JEONG;CHOI, SOO JUNG;SUH, YOUNG MIN;HEO, HO JIN |
分类号 |
A61K36/488;A61P25/28 |
主分类号 |
A61K36/488 |
代理机构 |
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