摘要 |
<p>A kit for genetically discriminating Angelica species is provided to improve accuracy of discrimination by discriminating relationship and genetic mutation among Angelica species, and maintain quality of Angelica species. The kit for genetically discriminating Angelica species comprises DNA extracting reagents, PCR reagents containing a sense primer of SEQ ID NO:1 and antisense primer of SEQ ID NO:2, and a sequencing primer of SEQ ID NO:3, wherein the Angelica species includes Angelica sinensis, Angelica acutiloba and Angelica gigas. The method for genetically discriminating Angelica species comprises the steps of: extracting DNA from an Angelica sample; preparing reaction solution of primers containing the sense and antisense primers and the extracted DNA; PCR-amplifying the reaction solution; sequencing the PCR product; and analyzing the genotypes of the amplified DNA by combining the amplified DNA and performing the pyrosequencing method.</p> |
申请人 |
INDUSTRY-ACADEMIC COORPERATION FOUNDATION DAEGU HAANY UNIVERSITY |
发明人 |
SEO, JUNG CHUL;LEEM, KANG HYUN;CHOI, HO YOUNG;CHOI, YOUNG JU;CHUNG, JOO HO;SHIN, DONG HOON;CHOI, KANG DUK;YANG, CHAE HA;KIM, SANG CHAN;HAN, SANG WON |