METHOD AND ALGORITHM FOR QUANTIFYING POLYNUCLEOTIDES

摘要

Machine executable method of analyzing growth curve data to identify the
transition from a baseline phase into a growth phase. Applications of the
method include analysis of results from time-dependent monitoring of amplicon
synthesis in a nucleic acid amplification reaction to quantify a starting
amount of a nucleic acid template in a test sample. The method advantageously
simplifies the quantitation by circumventing the need to establish thresholds
used for calculating initiation of the growth phase, to calculate derivatives,
or to perform linear regression analysis.