发明名称 |
High throughput assays for the proteolytic activities of clostridial neurotoxins |
摘要 |
In this application is described substrates for high-throughput assays of clostridial neurotoxin proteolytic activities. Two types of substrates are described for use in assays for the proteolytic activities of clostridial neurotoxins: (1) modified peptides or proteins that can serve as FRET substrates and (2) modified peptides or proteins that can serve as immobilized substrates. In both types a fluorescent molecules is present in the substrate, eliminating the requirement for the addition of a fluorigenic reagent. The assays described can be readily adapted for use in automated or robotic systems.
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申请公布号 |
US7157553(B2) |
申请公布日期 |
2007.01.02 |
申请号 |
US20050195098 |
申请日期 |
2005.08.02 |
申请人 |
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE ARMY |
发明人 |
SCHMIDT JAMES J.;STAFFORD ROBERT G. |
分类号 |
A61K38/00;C07K14/33;C12Q1/37;G01N33/542;G01N33/569;G01N33/68 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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