发明名称 |
ARABIDOPSIS-ORIGIN GDP-4-KETO-6-DEOXY-D-MANNOSE-3,5-EPIMERASE-4-REDUCTASE GENE |
摘要 |
The present invention relates to a gene for an enzyme involving in the synthesis of GDP-L-fucose. Particularly, the present invention relates to a GDP-4-keto-6-deoxy-D-mannose-3, 5-epimerase-4-reductase gene derived from Arabidopsis thaliana, and a process for producing GDP-L-fucose using the gene. An enzyme encoded by the gene is (a) a protein comprising an amino acid sequence represented by SEQ ID NO: 1; or (b) a protein comprising an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by deletion, substitution, addition or insertion of one or several amino acid residues, and having GDP-4-keto-6-deoxy-D-mannose-3, 5-epimerase-4-reductase activity. The present invention enables efficient mass production of GDP-L-fucose which is essential in performing addition of fucose, which has a very important function in sugar chains. |
申请公布号 |
EP1231266(B1) |
申请公布日期 |
2006.12.27 |
申请号 |
EP20000912999 |
申请日期 |
2000.03.30 |
申请人 |
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCEAND TECHNOLOGY |
发明人 |
NAKAYAMA, KEN-ICHI;JIGAMI, YOSHIFUMI |
分类号 |
C12N9/04;C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/02;C12N9/90;C12N15/53;C12N15/61;C12N15/63;C12P19/02;C12P19/32 |
主分类号 |
C12N9/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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