OSTEOBLAST COMPOSITION OF SEMI-SOLIDIFIED MIXED FIBRIN FOR BONE FRACTURE AGGLUTINATION AND ITS MANUFACTURING METHOD

摘要

A semi-solidified osteoblast composition for bone fracture agglutination comprising a mixture of an osteoblast and fibrin, and its manufacturing method are provided to induce fast bone fracture agglutination, uniformly distribute osteoblasts in the region to be treated, and avoid clinical rejection of the bone fracture agglutination by introducing the composition to the desired region of a subject. The method for manufacturing the semi-solidified osteoblast composition for bone fracture agglutination comprises the steps of: separating osteoblasts from the bone tissue, and culturing the osteoblasts in DMEM(Dulbecco's modified eagle's medium) or alpha-MEM(minimum essential medium, alpha modification) to prepare the osteoblast suspension; and adding a coagulant into the osteoblast suspension, wherein the coagulant is a mixture of thrombin and fibrinogen; and the amounts of thrombin and fibrinogen is 1-100 IU/ml and 20-100 mg/ml, respectively.