METHOD OF PROLIFERATING PLURIPOTENT STEM CELL

摘要

A novel method of proliferating pluripotent stem cells, such as ES cells. There are provided methods of proliferation and gene introduction for pluripotent stem cells, comprising culturing pluripotent stem cells in conditions maintaining their state of being unspecialized and differentiation-pluripotent, characterized in that by the use of a liquid culture medium and a culture vessel having a given concentration of molecules capable of adhering to the pluripotent stem cells applied to a substrate solid phase surface through immobilization or coating, proliferation or gene introduction/expression for the pluripotent stem cells is carried out in dispersed form while maintaining their state of being unspecialized and differentiation-pluripotent without the use of any feeder cells.