发明名称 |
IMMUNOGENE, NICHTGIFTIGE MUTANTEN DES CHOLERATOXINS UND DES TOXINS-LT, IHRE HERSTELLUNG UND IHRE VERWENDUNG ZUR HERSTELLUNG VON IMPFSTOFFEN |
摘要 |
An immunogenic detoxified protein comprising the amino acid sequence of subunit A of cholera toxin (CT-A) or subunit A of an Escherichia coli heat labile toxin (LT-A) or a fragment thereof wherein one or more amino acids at, or in positions corresponding to Val-53, Ser-63, Val-97, Tyr-104 or Pro-106 are replaced with another amino acid or deleted. Examples of specific replacements include Val-53-Asp, Val-53-Glu, Val-53-Tyr, Ser-63-Lys, Val-97-Lys, Val-97-Tyr, Tyr-104-Lys, Tyr-104-Asp, Tyr-104-Ser, Pro-106-Ser. The immunogenic detoxified protein is useful as vaccine for Vibrio cholerae or an enterotoxigenic strain of Escherichia coli and is produced by recombinant DNA means by site-directed mutagenesis. <IMAGE> |
申请公布号 |
AT346932(T) |
申请公布日期 |
2006.12.15 |
申请号 |
AT20040076971T |
申请日期 |
1992.12.30 |
申请人 |
NOVARTIS VACCINES AND DIAGNOSTICS S.R.L. |
发明人 |
DOMENIGHINI, MARIO;RAPPUOLI, RINO;PIZZA, MARIAGRAZIA;HOL, WIM |
分类号 |
C12N15/09;A61K38/00;A61K39/00;A61K39/106;A61K39/108;A61P31/04;C07K14/195;C07K14/245;C07K14/28;C07K14/41;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/31;C12P21/02;(IPC1-7):C12N15/31 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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