<p>The invention provides improved allelic ladder mixtures and individual alleles suitable for use in such mixtures, the allelic ladder mixture comprising one or more of the following allelic ladders:- an allelic ladder for locus D21S11 comprising one or more of alleles comprising or consisting of sequences :- (TCTA) 5 (TCTG) 6 (TCTA) 3 TCA (TCTA) 2 TCCATA (TCTA) 9 TCGTCT; (TCTA) 5 (TCTG) 6 (TCTA) 3 TCA (TCTA) 2 TCCATA (TCTA) 10 TCGTCT; (TCTA) 4 (TCTG) 6 (TCTA) 3 TA (TCTA) 3 TCA (TCTA) 2 TCCATA (TCTA) 8 TCGTCT; or at least 75% homologous thereto.</p>