A HEK 293 CELL LINE FOR HTS OF BLOCKERS AND BROAD-SPECTRUM STUDIES OF T-TYPE CALCIUM CHANNEL alpha;1I

摘要

A cell line is provided to provide high stable resting potential to HEK293 cell expressing T-type alpha II calcium channel so as to make a calcium signal through activation of the T-type alpha II calcium channel by highly concentrated KCl, thereby being efficiently used for searching a T-type alpha II calcium channel inhibitor. The cell line is characterized in that a gene coding potassium channel in a cell expressing T-type calcium alpha II calcium channel such as HEK 293 cell is transduced. In the cell line, the gene coding potassium channel is human Kir2.1 gene described as SEQ ID : NO. 1, has high stable resting potential, and is activated by being treated with potassium chloride or barium.