| 摘要 |
<p num="1"><br/><br/><br/>The present invention relates to the use of Stefin A as a scaffold protein for <br/>the display of inserted peptides, particularly wherein the Stefin A is a human <br/>Stefin A. Several mutations are advantageously made in the wild type stefin A <br/>sequence to improve it as a scaffold; preferably the Stefin A comprises a <br/>heterologous peptide insertion at the Leu 73 site. Furthermore, preferably the <br/>scaffold protein comprises a V48D mutation; preferably the scaffold protein <br/>comprises a G4W mutation. Preferably the scaffold comprises Leu73, V48D and <br/>G4W mutations. The invention also relates to the scaffold proteins themselves, <br/>in particular a stefin A polypeptide having the Leu73, V48D and G4W mutations, <br/>such as shown as SEQ ID NO: 1. The invention also relates to a method for <br/>identifying binding proteins and to peptide A (RLNKPLPSLPV) and its use in <br/>treating yeast infections.<br/>
|
