发明名称 |
Identification of genes altered in multiple myeloma |
摘要 |
<p>This invention provides a method of determining a chromosomal breakpoint in a subject suffering from multiple myeloma which comprises steps of: (a) obtaining a DNA sample from the subject suffering from multiple myeloma; (b) determining whether there is J and C disjunction in the immunoglobulin heavy chain gene in the obtained DNA sample; (c) obtaining a genomic library having clones which contain genomic DNA fragments from the DNA sample which shows positive J and C disjunction; (d) selecting and isolating clones of the obtained library which show positive hybridization with a probe which is capable of specifically hybridizing with the C but not the J region of the immunoglobulin heavy chain gene; (e) preparing fluorescent probes from the genomic DNA fragments of the isolated clones from step (d); (f) hybridizing said fluorescent probes with metaphase chromosomes; and (g) determining the identity of the chromosomes which are capable of hybridizing to said fluorescent probes, wherein the identification of a chromosome other than chromosome 14 would indicate that the chromosomal breakpoint is between chromosome 14 and the identified chromosome, thereby determining a chromosomal breakpoint in a subject suffering from multiple myeloma. This invention also provides the identified gene altered by a chromosomal breakpoint and various uses thereof.</p> |
申请公布号 |
EP1731144(A1) |
申请公布日期 |
2006.12.13 |
申请号 |
EP20060016570 |
申请日期 |
1997.05.28 |
申请人 |
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK |
发明人 |
DALLA-FAVERA, RICCARDO |
分类号 |
A61K31/00;C12N15/09;A61K31/70;A61K31/7088;A61K35/76;A61K36/00;A61K38/00;A61K48/00;A61P35/00;A61P35/02;C07H21/02;C07H21/04;C07K2/00;C07K14/435;C07K14/47;C07K14/82;C07K16/18;C07K16/32;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/00;C12N15/63;C12P21/02;C12P21/08;C12Q1/68 |
主分类号 |
A61K31/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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