摘要 |
Ester-form and free-form cholesterols contained in a very-low-density lipoprotein remnant (hereinafter, inclusively referred to as very-low- density lipoprotein remnant cholesterols) are determined. In an aqueous medium containing a sample, a cholesterol ester hydrolase and a cholesterol oxidase or cholesterol dehydrogenase are caused to act specifically on the very-low-density lipoprotein remnant cholesterols in the sample in the presence of a combination of (a) a polyoxyethylene- polyoxyalkylene alkylaryl ether and (b) at least one surfactant selected from the group consisting of a polyoxyethylene/polyoxybutylene condensate, a polyoxyethylene styrenated phenyl ether, and a polyoxyalkylene long- chain alkyl ether.
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