发明名称 |
Universalproben und Methoden zum Nachweis von Nukleinsäuren |
摘要 |
Signal primers are employed for detection of nucleic acid target sequences by fluorescence quenching mechanisms. The signal primer comprises a first and a second oligonucleotide and is partially single-stranded and partially double-stranded. In the presence of target, the second oligonucleotide of the signal primer is displaced from the first and a conformational change in a reporter probe occurs which changes the distance between the members of a donor/quencher dye pair linked to the reporter probe. The change in proximity between the dyes causes an increase or a decrease in fluorescence quenching, which is detected as an indication of the presence of the target sequence. |
申请公布号 |
DE60031489(D1) |
申请公布日期 |
2006.12.07 |
申请号 |
DE2000631489 |
申请日期 |
2000.08.23 |
申请人 |
BECTON, DICKINSON AND CO. |
发明人 |
NADEAU, JAMES G.;LINN, C. PRESTON;PITNER, J. BRUCE;DEAN, CHERYL H.;WALKER, TERRANCE G. |
分类号 |
C12Q1/68;G01N33/50;C12N15/09;G01N21/78;G01N33/566 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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