BINDING PARTNERS OF THE PLACENTAL GROWTH FACTOR, ESPECIALLY ANTIBODIES DIRECTED AGAINST THE PLACENTAL GROWTH FACTOR, PRODUCTION AND USE THEREOF

摘要

Immunologically active peptide (I), comprising sequence information of a primary placental growth factor isoform (PIGF), is new. Independent claims are included for: (1) a specific binding partner for PIGF, comprising specifically binding to the receptor binding domain on the secondary PIGF isoform; (2) an antigen binding fragment produced by specific binding partner; (3) a procedure for the production of the specific binding partner comprising immunizing an experimental animal with primary or secondary binding or non-binding (free) PIGF-isoform; fusing the spleen cells of the experimental animal with myeloma cell, where an antibody-producing hybrid cells develops; cloning the hybrid cells and selecting the hybrid cloned cell that produces an antibody selectively, which bind the receptor-binding domain of the secondary PIGF isoform; (4) an immunoassay to detect PIGF in a sample comprising contacting specifically binding partner or antigen binding fragment with the sample; and determining the formation of immune complex under participation of the PIGF qualitatively or quantitatively; (5) a test kit for carrying out immunoassays comprising specific binding partner or the antigen-binding fragment; and (6) a use of antibody and protein of the family receptor-tyrosine kinase, homologous, fragment or degradation in assaying the binding of the PIGF isoform.