| 摘要 |
The position of individual beads is automatically obtained and accurate fluorescence quantity is measured based on the obtained information in a bead chip array for which no array form information exists or whose array form information cannot be obtained in advance. When detecting fluorescence, bead reflected light is detected at the same time, so as to recognize the bead position. The reflected light from a bead can be detected in a similar manner for all beads, regardless of the presence or absence of a fluorescent substance. If the positions of all the beads are detected, accurate detection can be achieved by quantifying only the fluorescence at the detected positions. A fluorescent substance is caused to emit fluorescence using a light source having the absorption wavelength of the fluorescent substance. The fluorescence wavelength alone is detected by a first detector using a wavelength selection filter. Wavelengths other than the fluorescence wavelength are detected by a second detector, thereby obtaining the reflected light. Data on the thus obtained reflected light is processed into an image for obtaining the profile of the bead, the position of the bead is recognized by detecting the center position based on the profile, and the fluorescence is quantified based on the position of the bead. |
