发明名称 |
R2D2: an enzyme of RNA silencing |
摘要 |
Methods for making siRNA comprise recombinantly coexpressing a Dicer protein with an R2D2 protein to form a complex comprising the R2D2 protein and the Dicer protein, and contacting the complex with a double-stranded (ds) RNA under conditions wherein the complex cleaves the dsRNA into siRNA. The R2D2 protein may be a Drosophila R2D2 protein and the Dicer protein may be a Drosophila Dicer-2 protein. The Dicer protein and the R2D2 protein may be coexpressed in insect cells, such as S2, Sf9 or Hi5 cells, using a baculovirus expression system.
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申请公布号 |
US7144714(B2) |
申请公布日期 |
2006.12.05 |
申请号 |
US20030670626 |
申请日期 |
2003.09.25 |
申请人 |
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
WANG XIAODONG;LIU QINGHUA |
分类号 |
C12P19/34;C07H21/04;C07K14/435;C12N9/22;C12N15/11;C12P21/06;C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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