摘要 |
<p>The present invention relates to a process for preparing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane comprising contacting a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648 with (3S)-1-halo-2-oxo-3-(protected)amino-4-substituted butane substrate. The present invention also relates to a process comprising mixing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane with at least one base in the presence of at least one solvent to produce a reaction mixture containing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane. The present invention further relates to a process comprising crystallizing the (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane out of the reaction mixture by concurrently adding water and the reaction mixture together. The present invention is also directed to a mutagenized Rhodococcus erythropolis having ATCC deposit no. PTA-6648.</p> |
申请人 |
BRISTOL-MYERS SQUIBB COMPANY;BOWERS, NIGEL, IAN;SKONEZNY, PAUL, M.;STEIN, GREGORY, L.;FRANCESCHINI, THOMAS;CHIANG, SHU-JEN;ANDERSON, WENDY, L.;YOU, LI;XING, ZIZHUO |
发明人 |
BOWERS, NIGEL, IAN;SKONEZNY, PAUL, M.;STEIN, GREGORY, L.;FRANCESCHINI, THOMAS;CHIANG, SHU-JEN;ANDERSON, WENDY, L.;YOU, LI;XING, ZIZHUO |