OBESITY POLYPEPTIDE (OB) (VARIANTS), ITS ANALOGUE (VARIANTS), AND FUSED PROTEIN (VARIANTS), NUCLEIC ACID ISOLATED MOLECULE, DNA MOLECULE, CLONING RECOMBINANT VECTOR, EXPRESSION RECOMBINANT VECTOR, PHARMACEUTICAL COMPOSITION, MONOCLONAL AND POLYCLONAL ANTIBODY

摘要

FIELD: biotechnology, molecular biology, medicine. ^ SUBSTANCE: invention discloses amino acid sequences of human obesity polypeptide (OB) two isoforms possessing capacity for modulation of animal body mass, their signal peptide-containing precursors and analogues. Polypeptide isoforms are prepared as result of insertions, deletions and amino acid changes that retain activity typical for nonmodified forms of OB-polypeptides, and polyclonal and monoclonal antibodies interaction specifically with new agents modulating the body mass value also. Invention describes DNA sequences encoding these polypeptides and their analogues, vector structures comprising these sequences used for preparing recombinant forms of OB-polypeptides. Invention proposes using new polypeptides and their analogues as an active component in pharmaceutical compositions. Using this invention can promote to solving the problem for providing medicine, veterinary science and animal husbandry with effective agent used for decreasing the body mass value. Invention can be used in medicine for diagnosis and treatment of pathological states associated with disturbance of regulation of human body mass, and in animal husbandry and veterinary science. ^ EFFECT: valuable biological, medicinal and veterinary properties of polypeptide. ^ 23 cl, 71 dwg, 12 tbl, 17 ex