| 摘要 |
FIELD: biotechnology, biochemistry, amino acids, genetic engineering. ^ SUBSTANCE: invention relates to a method for producing L-threonine. Method involves partial inactivation of gene encoding enzyme threonine hydratase (tdc) in genome DNA of microorganism by using recombinant technology procedure. Strain of microorganism Escherichia coli pGm TN-PPC12 (KSMM-10236) is transformed with DNA fragment Deltatdc::1oxpKan and the strain E. coli TRN212 (KSSM-1053) is prepared. This strain is cultured for preparing L-threonine. The claimed invention provides significant increasing the yield of L-threonine. ^ EFFECT: valuable properties of strain. ^ 4 cl, 3 dwg, 4 tbl, 5 ex |
