FUSED TRANSCRIPT OF REARRANGED GENES SCCA1 AND SCCA2, DNA SEQUENCE ENCODING ITS, EXPRESSING PLASMID (VARIANTS)

摘要

FIELD: molecular biology, biochemistry, medicine, oncology. ^ SUBSTANCE: invention relates to DNA sequences found in analysis of mDNA from squamous carcinoma cellular lines of different origin wherein these DNA sequences represent transcripts from rearranged genes SCCA1 and SCCA2. Result of rearrangement is formation of fused gene consisting of exon 2-7 of gene SCCA1 and exon 8 of gene SCCA2, or exons 2-7 of gene SCCA2 and exon 8 of gene SCCA1. Prepared expressing vectors comprising above said combinations of exons of two genes provide synthesis of corresponding fused protein in host-cell. Proposed sequences of nucleic acids and genetic constructions based on thereof represent novel agents for diagnosis squamous carcinomas. ^ EFFECT: valuable biological and medicinal properties of transcripts. ^ 8 cl, 9 dwg, 1 tbl, 5 ex