METHOD OF PRODUCING INFECTIOUS REOVIRUS

摘要

PROBLEM TO BE SOLVED: To provide a simple and efficient method of producing a reovirus by culturing the reovirus in human embryo kidney 293 (HEK 293) cells.

SOLUTION: Disclosed is a method of producing a mammalian reovirus, comprising the steps of: (a) bringing human embryo kidney 293 (HEK 293) cells into contact with a mammalian reovirus and resulting in reoviral infection of the HEK 293 cells; (b) incubating the culture of the infected cells for a period of time sufficient to allow for viral replication; and (c) harvesting the virus produced.

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