| 摘要 |
Provided are a pH dependent ion exchange material which has high bonds to nucleic acid at first pH, releases nucleic acid with high rate at second pH, and has low bonds to protein, a solid substrate having the ion exchange material immobilized thereon, and a method for isolating nucleic acid by using the ion exchange material and the solid substrate. The pH dependent ion exchange material has carboxyl, amine groups, and polyethylene oxide moiety for isolating nucleic acid which comprise at least two monomers selected from the group consisting of the formulas M0, M1, M2, M3, and M4(the monomers are bonded with each other), and has at least one monomer selected from the group consisting of M1 and M2, and at least one monomers selected from the group consisting of M3 and M4. In the formulas, A is a base selected from -NH(CH2)nNH2(n is an integer of 1-10) and -NH(CH2)nY(n is an integer of 1-10, Y is an aromatic base in which at least one of cyclic elements are nitrogen), B is -(CH2CHO)nOR2(n is 1-20, R2 is an C1-C10 alkyl group or protecting group), R3 is a C1-C10 alkyl group. The ion exchange material has a polymerization degree of 2-30,000.
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