Molecule i.e. dipole, orientation determining method for e.g. cell, involves producing evanescent fields in sample by illuminating positions in lens pupil, where intensities at fluorescene correlate with different orientations of molecule

摘要

The method involves focusing an illumination light by a lens in a plane of a lens pupil. Differently oriented evanescent fields (9) are produced in a biological sample (10) e.g. cell, by illuminating different positions in the lens pupil, where intensities at fluorescene correlate with different orientations of a molecule i.e. dipole (13), in the sample. A laser light source is used for illumination of the sample. A light beam is positioned by a two dimensionally acting adjusting unit for illumination of the different positions in the lens pupil.