| 摘要 |
A novel Rhodococcus-E. coli shuttle vector comprising an origin of replication of a novel plasmid derived from Rhodococcus erythropolis IAM1484 and an origin of replication derived from E. coli, is disclosed. This shuttle vector replicates bidirectionally in E. coli and Rhodococcus. Also disclosed is a recombinant vector in which a target gene is operably linked to the shuttle vector, and microorganisms transformed with the recombinant vector. The shuttle vector is relatively small in size, can replicate in a variety of species of the genus Rhodococcus and has high replication stability in a host cell. Therefore, this shuttle vector is useful for cloning a variety of genes.
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