METHOD OF FLUORESCENT NANOSCOPY

摘要

The invention relates to analysis of object dyed with fluorescent colouring agents carried out with the aid of a fluorescent microscope. The microscope comprises an optical system for visualising and projecting a sample image to a video camera, a computer for recording and processing images, a sample holde r arranged in front of an object lens and a fluorescent radiation exciting source. The separately fluorescent visible molecules and nanoparticles are periodically formed in different object part. A laser produces the oscillati on thereof which is sufficient for recording the non-overlapping images of said molecules and nanoparticles and for decolouring already recorded fluorescent molecules. The pictures of recorded individual molecule and nanoparticle images are processed by a computer for searching the co-ordinates of the sta in centres and building the object image according to the calculated co-ordinat es of the stain centres corresponding to the co-ordinates of the individual fluorescent molecules and nanoparticles. Said invention makes it possible to obtain a two- and three-dimensional image with a resoling power better than 20 nm and to record a colour image by dyeing proteins, nucleic acids and lipids with different colouring agents.