ADJUVANT COMPRISING OLIGONUCLEOTIDE AND NON-TOXIC LPS

摘要

An immunoadjuvant comprising oligonucleotide and non-toxic lipopolysaccharide(LPS) is provided to improve safety, efficiency and specificity in induction of immune responses compared to using single of oligodeoxynucleotide immunoadjuvant by using bacteria-derived LPS. The immunoadjuvant composition comprises (a) oligodeoxynucleotide(ODN) and (b) non-toxic lipopolysaccharide(LPS) isolated from bacteria, wherein the oligodeoxynucleotide has more than 20 mer and optionally contains CG motif; the cytosine base of the oligodeoxynucleotide is optionally methylated; the oligodeoxynucleotide comprises the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3; the non-toxic polymer derived from lipopolysaccharide(LPS) has molecular weight of 2000 to 10000 dalton; the bacterium is Escherichia coli or Mycobacterium; and the immunoadjuvant composition is used as adjuvant of HBV(hepatitis B virus) vaccine.