| 摘要 |
<p>The invention relates to compositions and methods for detection of specific nucleic acid sequences during an amplification reaction. The invention further relates to a kit format of said compositions for detection of nucleic acid sequences. The Snapback oligonucleotide probes of the invention comprise a target binding sequence, a hairpin forming sequence, and a reporter binding sequence. The action of a nucleic acid polymerase or a flap endonuclease cleaves off a "snapback segment" of the probe containing the hairpin forming sequence and reporter binding sequence, causing the probe to "snap back" and form a hairpin structure. The further action of a nucleic acid polymerase or a flap endonuclease cleaves off a label moiety from a reporter oligonucleotide hybridized to the reporter binding sequence, resulting in a detectable signal.</p> |
