发明名称 |
METHOD FOR DETERMINING THE STATE OF ACTIVATION OF A PROTEIN |
摘要 |
<p>The present invention relates to a method for determining the state of activation of a (poly)peptide, comprising the steps of: (a) immobilizing a (poly)peptide on a carrier; (b) attaching the (poly)peptide immobilized on the carrier to a force measuring device of a force spectroscope; (c) applying a pulling force to the (poly)peptide and measuring the force required for stretching and/or unfolding of the (poly)peptide attached to the carrier, wherein the measurements are carried out (i) prior to and (ii) after treatment with a known modulator of said (poly)peptide; (d) comparing the force spectra of the (poly)peptide prior to and after treatment with the modulator; and (e) concluding from a difference of the force spectra of step (d) on the state of activation of the (poly)peptide.</p> |
申请公布号 |
WO2006120012(A1) |
申请公布日期 |
2006.11.16 |
申请号 |
WO2006EP04492 |
申请日期 |
2006.05.12 |
申请人 |
NAMBITION GMBH;KEDROV, ALEXEJ;MUELLER, DANIEL, J. |
发明人 |
KEDROV, ALEXEJ;MUELLER, DANIEL, J. |
分类号 |
G01Q10/00;G01Q60/24;G01Q60/42 |
主分类号 |
G01Q10/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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