摘要 |
The method for the culture of the fibroblasts and the myogenic cells of the newborn rats comprises the isolation of the substrate from skeletal and muscular tissue, the washing from the blood by Hanks solution containing 10 ?g/ml of Gentamycin, the mechanical chopping, the addition of the dispergation mixture (0.25% trypsin solution, versen solution, and Gentamycin), the washing, and the culture for 5-7 days.
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