| 摘要 |
A method of measuring the rate of binding of a binding substance and an analyte comprising disposing the binding substance and analyte in the medium, initially sonicating the mixture to disrupt binding between the binding substance and analyte and subsequently determining the rate of binding after cessation of the sonication. The binding may be immunological involving an antibody, antigen or a hapten and detected by optical means, including fluorescence. The sonication may be by ultrasound and the frequency may be at least 1kHZ, 20 kHZ or at a maximum of 1000 kHz. |
